1st published January 2013

A brief summary of MAF

MAF is a sugar protein that acts as a Macrophage Activating Factor (MAF) in the immune system.  It is part of the Active Vitamin D Molecule (1,25-dihydroxyvitamin D3), produced in the liver, is completely natural and the body expects it. Over 57 research papers have been published that demonstrate GcMAF activates Macrophages in both in vivo and in vitro studies

Macrophages – The ‘Big Eaters’

Macrophages function in both non-specific defense (innate immunity) as well as help initiate specific defence mechanisms (adaptive immunity) of vertebrate animals. Their role is to phagocytise (engulf and then digest) cellular debris and pathogens, either as stationary or as mobile cells. They also stimulate lymphocytes and other immune cells to respond to pathogens. They are specialized phagocytic cells that attack foreign substances, infectious microbes and cancer cells through destruction and ingestion.

Nagalase – collapsing the immune system.

In order to defeat the immune system and be able to grow and spread unchecked, cancers and many viruses secrete an enzyme called Nagalase which prevents the human body from producing its own MAF.  At a biological level the human body is constantly under attack so there is an excepted 'normal' level at 0.65 nmol/mg.  Levels above 0.65 nmol/mg indicate the immune system may have been compromised and the macrophages are not activated. 

85% of autistic children are found to have high levels of Nagalase.

Nagalase’s only purpose is to destroy GcMAF and this it does with great precision, time and time again with no other collateral damage. In this simple way Nagalase has been responsible for billions of deaths in human history.

MAF therapy

MAF is simply a protein replacement therapy, giving the body the protein it should be producing and expects. 

Dr. Yamamoto’s three studies showed that incredibly small weekly doses (100 billionths of a gram—an amount that is invisible to the naked eye) of GcMAF had cured early metastatic breast, prostate, and colon cancers in 100% of (non anaemic) patients. In a fourth paper, he used the same treatment to cure 100% of non anaemic HIV-infected patients.

More recently Dr Bradstreet & Dr Antonucci have performed MAF therapy on over 2000 autistic children with encouraging results.  Over 85% have responded favourably to MAF and 15% of these have lost the label of autism altogether

Nagalase Testing

A normal Nagalase level in a healthy adult is around 0.65 nmol/mg, and at this level the Nagalase does not interfere with the body’s own ability to generate GcMAF.  

Anybody with a reading of 1 or over may have a compromised immune system, but bear in mind that this enzyme fluctuates as viruses flourish and are beaten.  Two or more test may be necessary to overcome short term fluctuation. 

Nagalase was initially thought to be an indicator for cancer, but because it is generated by many other pathogens, it is a really an indicator of a depressed immune system.  However, the level of Nagalase does drop when cancer tumour burdens are reduced, and when viruses are defeated, so it is a good indicator of the beneficial interaction of exogenous GcMAF within immunotherapy.  

It is recommended that a Nagalase test is taken before commencing GcMAF and then monitored every 8 weeks so that the GcMAF therapy can be stopped when levels return to normal.

MAF and Autism

In August 2011, Dr Bradstreet, an autism specialist in the USA, made the discovery that his autistic patients have a higher than normal level of Nagalase.  Because Nagalase is not a test that is routinely done, he was the first person to make the connection that, perhaps, these patients were deficient in MAF.  

He then commenced a small scale trial of 40 patients, ranging in ages between 1 and 21.  The initial aim was to see if MAF would reduce the Nagalase levels.  The average Nagalase level reading for this group at the start of the trail was 1.93, and at the end of the trial period of 14 weeks that had reduced to 1.03.  

Apart from the likely immunological beneficial effects of lowering of Nagalase, substantial improvements in language, socialization and cognition were observed.  Using the CGI scale, a scale for psychiatric interventions, this showed improvements in 85% of patients.  40% showed dramatic improvements, with 15% going on to lose the label of autism.  


Whilst this report is not intended to be a nutritional guide, and for that a qualified nutritionalist should be sought, there are a few common sense guidelines that should help the efficacy of your immune system and MAF therapy.  

The MAF molecule is part of the larger Vitamin D process, and to work effectively, sufficient levels of vitamin D should be available.  The vitamin D that the body finds easiest to assimilate is that produced in sunlight on bare skin.  Vitamin D3 drops or tablets should be taken where sunlight is deficient, up to 5000 iu per day.  Vitamin D is a fat covered molecule so a healthy intake of good quality virgin oils such as olive, coconut or organic dairy produce will help the immune system.

Many autistic children suffer from intestinal problems, so a diet that excludes dairy and glucose products has been shown to offer relief from intestinal problems for many patients.  However, certain probiotic yoghurt types can help to rebalance the intestinal flora, and one has been developed that ferments out the lactose and casein elements during production and includes healthy bacterial strains that will encourage the activation of macrophages via the intestinal tract.  Good results have been seen by using the two variants of macrophage activation – the GcMAF and the maf yoghurt.

Using and Side effects of MAF

Autism is not limited to, but is the result of many viral and bacterial infections.   An awakened immune system will deal with these pathogens in no particular order.  Be aware, and make note of any immune responses such as low grade fevers and bacterial flare ups. These can be seen as a positive sign that the immune system is functioning. If the symptoms become aggravated then reduce the dosage of MAF. 

With autism, it is best to start at a low dose, these symptoms should never be more than a headache, low grade fever, or flu like symptoms.  

It is best to do this under the supervision of a doctor who is knowledgeable with MAF and its actions, but if this is not possible, there is a Facebook group called ‘GcMAF’ where many parents and patients share their knowledge and experiences.  

MAF – moving forward

Although over 3000 patients are now using MAF, the mechanism by which it helps autism is still not fully understood.  MAF is a complex and multi-faceted molecule, and much more than just a macrophage activating tool.  It turns cancer cells back to normal cells.  It activates the Vitamin D receptor pathways.  It improves bone density. It works as an actin scavenger, cleaning the body of toxins.  

The Nagalase improvements in the autism trial indicate that the viral load of the autistic patients decreases, so it is speculated that a reduction in the viral load creates a reduction in endotoxins which may explain some of the improvements in autistic behaviours.  

They simply now have a clearer head, similar to no longer being intoxicated.  In addition, there are three common alleles of GcProtein, and it may be that some variants of these may cause the GcMAF not to work on 15% of the population.  This requires further study, as all humans need GcMAF, but some may need a different variation. 

Useful References

GcMAF Book -  http://gcmaf.timsmithmd.com/

Dr Bradstreet Blog  http://drbradstreet.org/category/autism/

Dr Bradstreet Paper on Autism & Nagalase Initial Observations of Elevated Alpha-N-Acetylgalactosaminidase Activity Associated with Autism and Observed Reductions from GC Protein—Macrophage Activating Factor Injections

Dr Yamamoto Papers - http://www.gc-maf.de/en/the-yamamoto-papers.html

Dr Antonucci Video - http://vimeo.com/71554845



Nothing stated here should be considered as medical advice for dealing with a given problem. You should consult your Doctor for individual guidance for specific health problems. The information contained here is for informational and educational purposes only.


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